Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a gr...

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Main Authors: Hewitt, Stephen N., Choi, Ryan, Kelley, Angela, Crowther, Gregory J., Napuli, Alberto J., Van Voorhis, Wesley C.
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2011
Assuntos:
Laboratory Communications
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169393/
https://ncbi.nlm.nih.gov/pubmed/21904041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111022159
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