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Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag

Expression of fusion proteins such as MBP fusions can be used as a way to improve the solubility of the expressed protein in E. coli (Fox and Waugh, 2003; Nallamsetty et al., 2005; Nallamsetty and Waugh, 2006) and as a way to introduce an affinity purification tag. The protocol that follows was desi...

詳細記述

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書誌詳細
出版年:Methods Enzymol
主要な著者: Duong-Ly, Krisna C., Gabelli, Sandra B.
フォーマット: Artigo
言語:Inglês
出版事項: 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4612625/
https://ncbi.nlm.nih.gov/pubmed/26096500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.004
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