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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein
Sixteen of 22 low molecular weight integral membrane proteins from Mycobacterium tuberculosis with previously poor or undetectable levels of expression were expressed in Escherichia coli as fusions with both the maltose-binding protein (MBP) and a His(8)-tag. 68% of targeted proteins were expressed...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2006
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2684689/ https://ncbi.nlm.nih.gov/pubmed/17275326 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.022 |
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