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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

Sixteen of 22 low molecular weight integral membrane proteins from Mycobacterium tuberculosis with previously poor or undetectable levels of expression were expressed in Escherichia coli as fusions with both the maltose-binding protein (MBP) and a His(8)-tag. 68% of targeted proteins were expressed...

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Detalhes bibliográficos
Main Authors: KOREPANOVA, A., MOORE, J.D., NGUYEN, H., HUA, Y., CROSS, T. A., GAO, F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684689/
https://ncbi.nlm.nih.gov/pubmed/17275326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.022
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