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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

Sixteen of 22 low molecular weight integral membrane proteins from Mycobacterium tuberculosis with previously poor or undetectable levels of expression were expressed in Escherichia coli as fusions with both the maltose-binding protein (MBP) and a His(8)-tag. 68% of targeted proteins were expressed...

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書誌詳細
主要な著者: KOREPANOVA, A., MOORE, J.D., NGUYEN, H., HUA, Y., CROSS, T. A., GAO, F.
フォーマット: Artigo
言語:Inglês
出版事項: 2006
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684689/
https://ncbi.nlm.nih.gov/pubmed/17275326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.022
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