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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

Sixteen of 22 low molecular weight integral membrane proteins from Mycobacterium tuberculosis with previously poor or undetectable levels of expression were expressed in Escherichia coli as fusions with both the maltose-binding protein (MBP) and a His(8)-tag. 68% of targeted proteins were expressed...

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Bibliografiske detaljer
Main Authors: KOREPANOVA, A., MOORE, J.D., NGUYEN, H., HUA, Y., CROSS, T. A., GAO, F.
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684689/
https://ncbi.nlm.nih.gov/pubmed/17275326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.022
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