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Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SS...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3169392/ https://ncbi.nlm.nih.gov/pubmed/21904040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111017374 |
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