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Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells
Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intens...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2006
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3169331/ https://ncbi.nlm.nih.gov/pubmed/16793282 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2006.04.004 |
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