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Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells
Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intens...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3169331/ https://ncbi.nlm.nih.gov/pubmed/16793282 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2006.04.004 |
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