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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

Transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. In case of enzymes, for example, transien...

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Detalhes bibliográficos
Principais autores: Biro, F. Noah, Zhai, Jie, Doucette, Christopher W., Hingorani, Manju M.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3168206/
https://ncbi.nlm.nih.gov/pubmed/20357752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1874
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