Frozen Tissue Can Provide Reproducible Proteomic Results of Subcellular Fractionation

Differential detergent fractionation (DDF) is frequently used to partition fresh cells and tissues into distinct compartments. We have tested whether DDF can reproducibly extract and fractionate cellular protein components from frozen tissues. Frozen kidneys were sequentially extracted with three di...

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Detaylı Bibliyografya
Asıl Yazarlar: Lim, Jihyeon, Menon, Vilas, Bitzer, Markus, Miller, Leah M., Madrid-Aliste, Carlos, Weiss, Louis M., Fiser, Andras, Angeletti, Ruth H.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2011
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3164751/
https://ncbi.nlm.nih.gov/pubmed/21802400
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.045
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