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Frozen Tissue Can Provide Reproducible Proteomic Results of Subcellular Fractionation

Differential detergent fractionation (DDF) is frequently used to partition fresh cells and tissues into distinct compartments. We have tested whether DDF can reproducibly extract and fractionate cellular protein components from frozen tissues. Frozen kidneys were sequentially extracted with three di...

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Detalhes bibliográficos
Main Authors: Lim, Jihyeon, Menon, Vilas, Bitzer, Markus, Miller, Leah M., Madrid-Aliste, Carlos, Weiss, Louis M., Fiser, Andras, Angeletti, Ruth H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3164751/
https://ncbi.nlm.nih.gov/pubmed/21802400
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.045
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