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Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells

BACKGROUND: The isolation of DNA-free RNA is a crucial step in the reverse transcription PCR (RT-PCR). Every RNA extraction procedure results in RNA samples contaminated with genomic DNA, which can cause false-positive outcomes in highly sensitive applications, including a recently developed quantit...

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Bibliografiske detaljer
Main Authors: Rump, Lydia V, Asamoah, Benedicta, Gonzalez-Escalona, Narjol
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3161365/
https://ncbi.nlm.nih.gov/pubmed/20663210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-211
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