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A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

BACKGROUND: This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tissues (spleen, blood, kidney, embryo, and larvae) using the Qiagen RNeasy®...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Sellin Jeffries, Marlo K, Kiss, Andor J, Smith, Austin W, Oris, James T
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4239376/
https://ncbi.nlm.nih.gov/pubmed/25394494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-014-0094-8
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