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Evaluation of FKBP and DHFR based destabilizing domains in Saccharomyces cerevisiae
Two orthogonal destabilizing domains have been developed based on mutants of human FKBP12 as well as bacterial DHFR and these engineered domains have been used to control protein concentration in a variety of contexts in vitro and in vivo. FKBP12 based destabilizing domains can not be rescued in the...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2011
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3156383/ https://ncbi.nlm.nih.gov/pubmed/21741238 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2011.06.006 |
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