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Synthesis and analysis of stabilizing ligands for FKBP-derived destabilizing domains

We recently identified mutants of the human FKBP12 protein that are unstable and rapidly degraded when expressed in mammalian cells. We call these FKBP mutants destabilizing domains (DDs), because their instability is conferred to any protein fused to the DDs. A cell-permeable ligand binds tightly t...

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Detalhes bibliográficos
Main Authors: Grimley, Joshua S., Chen, Denise A., Banaszynski, Laura A., Wandless, Thomas J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2245802/
https://ncbi.nlm.nih.gov/pubmed/18039574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2007.11.044
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