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Synthesis and analysis of stabilizing ligands for FKBP-derived destabilizing domains

We recently identified mutants of the human FKBP12 protein that are unstable and rapidly degraded when expressed in mammalian cells. We call these FKBP mutants destabilizing domains (DDs), because their instability is conferred to any protein fused to the DDs. A cell-permeable ligand binds tightly t...

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Detalles Bibliográficos
Main Authors: Grimley, Joshua S., Chen, Denise A., Banaszynski, Laura A., Wandless, Thomas J.
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2245802/
https://ncbi.nlm.nih.gov/pubmed/18039574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2007.11.044
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