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Detection of 5-Hydroxymethylcytosine in a Combined Glycosylation Restriction Analysis (CGRA) Using Restriction Enzyme Taq(α)I
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA base in mammalian cells that is believed to be another important epigenetic modification. Here we report the use of a methylation-insensitive restriction enzyme Taq(α)I coupled with selective chemical labeling of 5-hmC in a Combined Glycosyla...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2011
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3156281/ https://ncbi.nlm.nih.gov/pubmed/21524909 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2011.03.118 |
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