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TIME RESOLVED EVENTS ON THE REACTION PATHWAY OF TRANSCRIPT INITIATION BY A SINGLE SUBUNIT RNA POLYMERASE: RAMAN CRYSTALLOGRAPHIC EVIDENCE
The nucleotidyl transfer reaction leading to formation of the first phosphodiester bond has been followed in real-time by Raman microscopy, as it proceeds in single crystals of the N4 phage virion RNA polymerase (RNAP). The reaction is initiated by soaking NTP substrates and divalent cations into th...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3154994/ https://ncbi.nlm.nih.gov/pubmed/21744806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja201557w |
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