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A Comparison of Exogenous Promoter Activity at the ROSA26 Locus Using a PhiC31 Integrase Mediated Cassette Exchange Approach in Mouse ES Cells

The activities of nine ubiquitous promoters (ROSA26, CAG, CMV, CMVd1, UbC, EF1α, PGK, chicken β-actin and MC1) have been quantified and compared in mouse embryonic stem cells. To avoid the high variation in transgene expression which results from uncontrolled copy number and chromosomal position eff...

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Detalhes bibliográficos
Main Authors: Chen, Chiann-mun, Krohn, Jon, Bhattacharya, Shoumo, Davies, Benjamin
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3154917/
https://ncbi.nlm.nih.gov/pubmed/21853122
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0023376
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