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Preparation and Ultrastructure of the Outer Coats of Azotobacter vinelandii Cysts

Cysts of Azotobacter vinelandii were ruptured with 3.0 mm ethylenediaminetetraacetate in 0.05 m tris(hydroxymethyl)aminomethane buffer (pH 7.8) and fractionated by differential centrifugation in density gradients of sucrose or Ficoll (polysucrose) at 10,000 × g for 2 hr at 4 C. The average densities...

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Detalhes bibliográficos
Main Authors: Lin, L. P., Sadoff, H. L.
Formato: Artigo
Idioma:Inglês
Publicado em: 1969
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC315331/
https://ncbi.nlm.nih.gov/pubmed/4977988
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