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Preparation and Ultrastructure of the Outer Coats of Azotobacter vinelandii Cysts
Cysts of Azotobacter vinelandii were ruptured with 3.0 mm ethylenediaminetetraacetate in 0.05 m tris(hydroxymethyl)aminomethane buffer (pH 7.8) and fractionated by differential centrifugation in density gradients of sucrose or Ficoll (polysucrose) at 10,000 × g for 2 hr at 4 C. The average densities...
Gardado en:
| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1969
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC315331/ https://ncbi.nlm.nih.gov/pubmed/4977988 |
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