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A fluorescent assay to quantitatively measure in vitro acyl CoA:diacylglycerol acyltransferase activity
Triacylglycerols (TG) are the major storage form of energy in eukaryotic organisms and are synthesized primarily by acyl CoA:1,2-diacylglycerol acyltransferase (DGAT) enzymes. In vitro DGAT activity has previously been quantified by measuring the incorporation of either radiolabeled fatty acyl CoA o...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Biochemistry and Molecular Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3151697/ https://ncbi.nlm.nih.gov/pubmed/21653930 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D016626 |
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