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Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells

Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving unifo...

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Détails bibliographiques
Auteurs principaux: Khandelia, Piyush, Yap, Karen, Makeyev, Eugene V.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3150880/
https://ncbi.nlm.nih.gov/pubmed/21768390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1103532108
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