Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ancient bones. The method greatly reduces sample destruction and sequencing demands relative to direct PCR or shotgun sequencing approaches. We use...

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Bibliographic Details
Main Authors: Briggs, Adrian W., Good, Jeffrey M., Green, Richard E., Krause, Johannes, Maricic, Tomislav, Stenzel, Udo, Pääbo, Svante
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2009
Subjects:
Cellular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3150061/
https://ncbi.nlm.nih.gov/pubmed/19730410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1573
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