Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides

Immunoassays are routinely used as research tools to measure intracellular cAMP and cGMP concentrations. Ideally, this application requires antibodies with high sensitivity and specificity. The present work evaluates the cross-reactivity of commercially available cyclic nucleotide analogs with two n...

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Bibliographic Details
Main Authors: Werner, Katharina, Schwede, Frank, Genieser, Hans-Gottfried, Geiger, Jörg, Butt, Elke
Format: Artigo
Language:Inglês
Published: Springer-Verlag 2011
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Original Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3145891/
https://ncbi.nlm.nih.gov/pubmed/21713381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00210-011-0662-6
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