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Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides

Immunoassays are routinely used as research tools to measure intracellular cAMP and cGMP concentrations. Ideally, this application requires antibodies with high sensitivity and specificity. The present work evaluates the cross-reactivity of commercially available cyclic nucleotide analogs with two n...

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Bibliografske podrobnosti
Main Authors: Werner, Katharina, Schwede, Frank, Genieser, Hans-Gottfried, Geiger, Jörg, Butt, Elke
Format: Artigo
Jezik:Inglês
Izdano: Springer-Verlag 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3145891/
https://ncbi.nlm.nih.gov/pubmed/21713381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00210-011-0662-6
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