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Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides

Immunoassays are routinely used as research tools to measure intracellular cAMP and cGMP concentrations. Ideally, this application requires antibodies with high sensitivity and specificity. The present work evaluates the cross-reactivity of commercially available cyclic nucleotide analogs with two n...

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Detalhes bibliográficos
Main Authors: Werner, Katharina, Schwede, Frank, Genieser, Hans-Gottfried, Geiger, Jörg, Butt, Elke
Formato: Artigo
Idioma:Inglês
Publicado em: Springer-Verlag 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3145891/
https://ncbi.nlm.nih.gov/pubmed/21713381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00210-011-0662-6
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