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EPR SPECTRA AND MOLECULAR DYNAMICS AGREE THAT THE NUCLEOTIDE POCKET OF MYOSIN V IS CLOSED AND THAT IT OPENS ON BINDING ACTIN

We have used EPR spectroscopy and computational modeling of nucleotide-analog spin probes to investigate conformational changes at the nucleotide site of myosin V (MV). We find that in the absence of actin, the mobility of a spin-labeled diphosphate analog (SLADP) bound at the active site is strongl...

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Detalhes bibliográficos
Main Authors: Purcell, Thomas J., Naber, Nariman, Sutton, Shirley, Cooke, Roger, Pate, Edward
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3143297/
https://ncbi.nlm.nih.gov/pubmed/21640122
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.05.019
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