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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA template is attached to a functionalized glass coverslip and replicated extensively after introduction of replication proteins and nucleotides (Figur...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Tanner, Nathan A., Loparo, Joseph J., van Oijen, Antoine M.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3142874/
https://ncbi.nlm.nih.gov/pubmed/19820685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1529
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