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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA template is attached to a functionalized glass coverslip and replicated extensively after introduction of replication proteins and nucleotides (Figur...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MyJove Corporation
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3142874/ https://ncbi.nlm.nih.gov/pubmed/19820685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1529 |
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