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Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection...

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Detalhes bibliográficos
Main Authors: Konry, Tania, Dominguez-Villar, Margarita, Baecher-Allan, Clare, Hafler, David A., Yarmush, Martin. L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3141325/
https://ncbi.nlm.nih.gov/pubmed/20888750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bios.2010.09.006
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