Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy

OBJECTIVE: Recent publications questioned the validity of endothelial cell (EC) culture studies of glycocalyx (GCX) function, due to findings that GCX in vitro may be substantially thinner than in vivo. The assessment of thickness differences is complicated by GCX collapse during dehydration for tra...

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Bibliographic Details
Main Authors: Ebong, Eno E, Macaluso, Frank P, Spray, David C, Tarbell, John M
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3141106/
https://ncbi.nlm.nih.gov/pubmed/21474821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/ATVBAHA.111.225268
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