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Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy

OBJECTIVE: Recent publications questioned the validity of endothelial cell (EC) culture studies of glycocalyx (GCX) function, due to findings that GCX in vitro may be substantially thinner than in vivo. The assessment of thickness differences is complicated by GCX collapse during dehydration for tra...

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Bibliografische gegevens
Hoofdauteurs: Ebong, Eno E, Macaluso, Frank P, Spray, David C, Tarbell, John M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2011
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3141106/
https://ncbi.nlm.nih.gov/pubmed/21474821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/ATVBAHA.111.225268
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