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Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-fri...
Gespeichert in:
| Veröffentlicht in: | J Vis Exp |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MyJove Corporation
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4692431/ https://ncbi.nlm.nih.gov/pubmed/25350384 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51844 |
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