Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-fri...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Bobik, Krzysztof, Dunlap, John R., Burch-Smith, Tessa M.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2014
Subjects:
Plant Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692431/
https://ncbi.nlm.nih.gov/pubmed/25350384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51844
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