Reengineering Rate-Limiting, Millisecond Enzyme Motions by Introduction of an Unnatural Amino Acid

Rate-limiting millisecond motions in wild-type (WT) Ribonuclease A (RNase A) are modulated by histidine 48. Here, we incorporate an unnatural amino acid, thia-methylimidazole, at this site (H48C-4MI) to investigate the effects of a single residue on protein motions over multiple timescales and on en...

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Bibliografiske detaljer
Main Authors: Watt, Eric D., Rivalta, Ivan, Whittier, Sean K., Batista, Victor S., Loria, J. Patrick
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2011
Fag:
Protein
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136797/
https://ncbi.nlm.nih.gov/pubmed/21767494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.05.039
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