Reengineering Rate-Limiting, Millisecond Enzyme Motions by Introduction of an Unnatural Amino Acid

Rate-limiting millisecond motions in wild-type (WT) Ribonuclease A (RNase A) are modulated by histidine 48. Here, we incorporate an unnatural amino acid, thia-methylimidazole, at this site (H48C-4MI) to investigate the effects of a single residue on protein motions over multiple timescales and on en...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Watt, Eric D., Rivalta, Ivan, Whittier, Sean K., Batista, Victor S., Loria, J. Patrick
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Biophysical Society 2011
Schlagworte:
Protein
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136797/
https://ncbi.nlm.nih.gov/pubmed/21767494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.05.039
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge