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Stabilization of hypoxia-inducible factor-1α in buffer containing cobalt chloride for Western blot analysis
Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to stabilize HIF-1α protein during sample preparation for Western blot analysis due to its rapid degradation in the presence of oxygen. Enzyme inhibitor cocktails can be complex and ex...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3125494/ https://ncbi.nlm.nih.gov/pubmed/21601556 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.04.037 |
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