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Directed Evolution of a Small Molecule-Triggered Intein with Improved Splicing Properties in Mammalian Cells
Laboratory-created small-molecule-dependent inteins enable protein structure and function to be controlled post-translationally in living cells. Previously we evolved two inteins (2-4 and 3-2) that splice efficiently in the presence, but not the absence, of the cell-permeable small molecule 4-hydrox...
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| Huvudupphovsmän: | , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2011
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3124510/ https://ncbi.nlm.nih.gov/pubmed/21609843 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.02.014 |
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