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Improved protein splicing using embedded split inteins

Naturally split inteins mediate a traceless protein ligation process known as protein trans‐splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fra...

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書目詳細資料
發表在:Protein Sci
Main Authors: Gramespacher, Josef A., Stevens, Adam J., Thompson, Robert E., Muir, Tom W.
格式: Artigo
語言:Inglês
出版: John Wiley and Sons Inc. 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818749/
https://ncbi.nlm.nih.gov/pubmed/29226478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3357
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