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Improved protein splicing using embedded split inteins

Naturally split inteins mediate a traceless protein ligation process known as protein trans‐splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fra...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Gramespacher, Josef A., Stevens, Adam J., Thompson, Robert E., Muir, Tom W.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818749/
https://ncbi.nlm.nih.gov/pubmed/29226478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3357
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