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Improved protein splicing using embedded split inteins
Naturally split inteins mediate a traceless protein ligation process known as protein trans‐splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fra...
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| Published in: | Protein Sci |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
John Wiley and Sons Inc.
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5818749/ https://ncbi.nlm.nih.gov/pubmed/29226478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3357 |
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