Improved protein splicing using embedded split inteins

Naturally split inteins mediate a traceless protein ligation process known as protein trans‐splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fra...

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Bibliographic Details
Published in:Protein Sci
Main Authors: Gramespacher, Josef A., Stevens, Adam J., Thompson, Robert E., Muir, Tom W.
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2018
Subjects:
Accelerated Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818749/
https://ncbi.nlm.nih.gov/pubmed/29226478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3357
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