Functional and Metabolic Effects of Adaptive Glycerol Kinase (GLPK) Mutants in Escherichia coli

Herein we measure the effect of four adaptive non-synonymous mutations to the glycerol kinase (glpK) gene on catalytic function and regulation, to identify changes that correlate to increased fitness in glycerol media. The mutations significantly reduce affinity for the allosteric inhibitor fructose...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Applebee, M. Kenyon, Joyce, Andrew R., Conrad, Tom M., Pettigrew, Donald W., Palsson, Bernhard Ø.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2011
Soggetti:
Enzymology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3123082/
https://ncbi.nlm.nih.gov/pubmed/21550976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.195305
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi