Artificial steroid hormone response element generated by dam-methylation.
Using the interaction of steroid hormone receptors with their palindromic response elements as an example, we show here that cloning in dam+ bacterial strains can lead to artifactual results due to methylation of adenine residues at the N-6 position. Substitution of the T by an A in the third positi...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Nucleic Acids Res |
|---|---|
| Prif Awduron: | , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
Oxford University Press
1992
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| Mynediad Ar-lein: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312226/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1579439/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.7.1483 |
| Tagiau: |
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