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Artificial steroid hormone response element generated by dam-methylation.

Using the interaction of steroid hormone receptors with their palindromic response elements as an example, we show here that cloning in dam+ bacterial strains can lead to artifactual results due to methylation of adenine residues at the N-6 position. Substitution of the T by an A in the third positi...

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Detalhes bibliográficos
Main Authors: Truss, M, Bartsch, J, Chalepakis, G, Beato, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC312226/
https://ncbi.nlm.nih.gov/pubmed/1579439
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