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Artificial steroid hormone response element generated by dam-methylation.
Using the interaction of steroid hormone receptors with their palindromic response elements as an example, we show here that cloning in dam+ bacterial strains can lead to artifactual results due to methylation of adenine residues at the N-6 position. Substitution of the T by an A in the third positi...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1992
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312226/ https://ncbi.nlm.nih.gov/pubmed/1579439 |
| Tags: |
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