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Insertion of the Designed Helical Linker Led to Increased Expression of Tf- based fusion proteins
PURPOSE: To demonstrate a high-level expression of transferrin (Tf)-based fusion proteins by inserting a helical linker between two protein domains. METHODS: Tf-based fusion proteins were designed to contain oligonucleotides encoding a helical linker inserted between the protein domains. Plasmid con...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3121561/ https://ncbi.nlm.nih.gov/pubmed/19002568 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11095-008-9767-0 |
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