Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol.

Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. Similar stimulation was also caused by divalent cations or p...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1986
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3022231/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.19.7617
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