Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol.
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. Similar stimulation was also caused by divalent cations or p...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1986
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3022231/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.19.7617 |
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