Stimulation of intermolecular ligation with E. coli DNA ligase by high concentrations of monovalent cations in polyethylene glycol solutions.
In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt concentrations: 200 mM NaCl or 300 mM KCl in 10% (w/v) PEG 6000 solutions, and 100-200 mM NaCl or 15...
Na minha lista:
| Publicado no: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1985
|
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322104/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3906565/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.7979 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|