C4-methyldeoxythymidine replacing deoxythymidine in poly[d(A-T)] renders the polymer resistant to the 3'----5' exonuclease activity of the Klenow and T4 DNA polymerases.
We previously reported that O4-alkyl dTTPs could replace, for short times, dTTP in polymer synthesis [Singer et al., PNAS 83, 26-32, 1986]. The reasons for such early termination of synthesis could be either proofreading or the eventual formation of weakly paired primer termini. Utilizing the known...
Salvato in:
| Pubblicato in: | Nucleic Acids Res |
|---|---|
| Autore principale: | |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1986
|
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311677/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3529040/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6735 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|