C4-methyldeoxythymidine replacing deoxythymidine in poly[d(A-T)] renders the polymer resistant to the 3'----5' exonuclease activity of the Klenow and T4 DNA polymerases.
We previously reported that O4-alkyl dTTPs could replace, for short times, dTTP in polymer synthesis [Singer et al., PNAS 83, 26-32, 1986]. The reasons for such early termination of synthesis could be either proofreading or the eventual formation of weakly paired primer termini. Utilizing the known...
Zapisane w:
| Wydane w: | Nucleic Acids Res |
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| 1. autor: | |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Oxford University Press
1986
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| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311677/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3529040/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6735 |
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