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High-Yield Expression of a Catalytically Active Membrane-Bound Protein: Human P450 Oxidoreductase
P450 oxidoreductase (POR) is a two-flavin protein that reduces microsomal P450 enzymes and some other proteins. Preparation of active bacterially expressed human POR for biochemical studies has been difficult because membrane-bound proteins tend to interact with column matrices. To reduce column-pro...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Endocrine Society
2011
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3115607/ https://ncbi.nlm.nih.gov/pubmed/21586563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1210/en.2011-0230 |
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