Secondary structure as primary determinant of the efficiency of ribosomal binding sites in Escherichia coli.

Using a previously described vector (pKL203) we fused several heterologous ribosomal binding sites (RBSs) to the lacZ gene of E. coli and then studied the variation in expression of the fusions. The RBSs originated from bacteriophage Q beta and MS2 genes and the E. coli genes for elongation factor E...

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Bibliographic Details
Main Authors: Looman, A C, Bodlaender, J, de Gruyter, M, Vogelaar, A, van Knippenberg, P H
Format: Artigo
Language:Inglês
Published: 1986
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC311554/
https://ncbi.nlm.nih.gov/pubmed/3526283
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