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Engineering streptokinase for generation of active site-labeled plasminogen analogs

We previously demonstrated that streptokinase (SK) can be used to generate active site-labeled fluorescent analogs of plasminogen (Pg) by virtue of its non-proteolytic activation of the zymogen. The method is versatile and allows for stoichiometric and active site-specific incorporation of any one o...

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Autors principals: Laha, Malabika, Panizzi, Peter, Nahrendorf, Matthias, Bock, Paul E.
Format: Artigo
Idioma:Inglês
Publicat: 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3114107/
https://ncbi.nlm.nih.gov/pubmed/21570944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.04.025
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